Rb What Do You Make Of This--oleate Helps Survival Of Bc Cell Lines Even Wout Serum!

[RobinP]

I wonder if these in vitro studies were on just bc cell lines that were only er sensitive ones, as there is a lot of evidence from Dr Menendez that oleic acid has efficacy in her2 bc.

[R.B.]

Robin,


From memory I think the MDA cell line is an HER 2 line, but I am not certain.

And yes Dr Martinez is pretty definitive in a few trials. He also suggests ensuring adequate supplies of threes and nines.

Fats work in so many different ways, and impact the cell metabolism from a number of angles, and it is so complex I think it is difficult to reach any more thoughts without access to the trial, which is a pay for view only.

We are also looking at it out of context of the body and real world in the sense that it may work by displacing something else like say linoleic acid (omega six), and we don't know if that figured here.

At a basic level I think it comes back to supplying the body with the raw materials it has grown up with, taking into account that most people will have omega six stores that will take a while to shift, and many have blocked fat conversion pathways to make DHA etc.

E.g. more three than six and some long chain threes, keeping an eye out for long chain omega six deficiency in a few. The point being for most our modern diets do not supply us with what we need by a long way.

I do have some questions and thoughts on the above but they are too hazy to post.

Please all discuss dietary change with your doctor. This is information and not advice.

RB

[RobinP]

MDA line may lack estrogen; however, it may also lack her2. Perhaps thats why it didn't respond oleic acid. I also think the in vivo impact may differ than the in vitro... we certainly lack human studies when it comes to the cancer diet. Like I said before, the impact of particular fatty acids is much more defined for the cardiac diet.

[Christine MH-UK]

Just did a quick check through pubmed. Clearest example: "Medium dose-effect analysis indicates that OSU-03012 potentiated trastuzumab's antiproliferative effect in HER2-positive cells, especially in SKBR3/IGF-IR cells, through the down-regulation of PDK-1/Akt signaling. This synergy, however, was not observed in HER2-negative MDA-MB-231 cells."

[R.B.]

Thanks Christine for taking the trouble to post this.

I just wondered if there might be other strains of MDA-MB-231 as well.

Also in the cycle of breaking and remaking referred to "Rapid TG lipolysis and FFA re-esterification" what fats are being made. Are the cells making the long chain omega nines??.

RB