Putative genomics of the mechanism of action of the olive oil "Meditteranean diet"

1: Eur J Cancer. 2006 Jan 4; [Epub ahead of print] Links

A genomic explanation connecting "Mediterranean diet", olive oil and cancer: Oleic acid, the main monounsaturated Fatty acid of olive oil, induces formation of inhibitory "PEA3 transcription factor-PEA3 DNA binding site" complexes at the Her-2/neu (erbB-2) oncogene promoter in breast, ovarian and stomach cancer cells.

Menendez JA, Papadimitropoulou A, Vellon L, Colomer R, Lupu R.

Department of Medicine, Evanston Northwestern Healthcare Research Institute, 1001 University Place, Evanston, IL 60201, USA; Department of Medicine, Northwestern University Feinberg, School of Medicine, Chicago, IL, USA; Robert H. Lurie Comprehensive Cancer Center of Northwestern University, Chicago, IL, USA.

Olive oil is an integral ingredient of the "Mediterranean diet" and accumulating evidence suggests that it may have a potential role in lowering risk of several cancers. We recently hypothesized that the anti-cancer actions of olive oil may relate to its monounsaturated fatty acid (MUFA) oleic acid (OA; 18:1n-9) content to specifically regulate oncogenes. In this study, transient transfection experiments with human Her-2/neu promoter-driven luciferase gene established the ability of OA to specifically repress the transcriptional activity of Her-2/neu gene. Gene repression was seen in tumour-derived cell lines with Her-2/neu gene amplification and overexpression, including SK-Br3 (56% reduction), SK-OV3 (75% reduction) and NCI-N87 (55% reduction) breast, ovarian and stomach cancer cell lines, respectively. Also marginal decreases in promoter activity were observed in cancer cells expressing physiological levels of Her-2/neu (20% reduction in MCF-7 breast cancer cells). Remarkably, OA treatment in Her-2/neu-overexpressing cancer cells was found to induce up-regulation of the Ets protein polyomavirus enhancer activator 3 (PEA3), a transcriptional repressor of Her-2/neu promoter. Also, an intact PEA3 DNA-binding-site at endogenous Her-2/neu gene promoter was essential for OA-induced repression of this gene. Moreover, OA treatment failed to decrease Her-2/neu protein levels in MCF-7/Her2-18 transfectants, which stably express full-length human Her-2/neu cDNA controlled by a SV40 viral promoter. OA-induced transcriptional repression of Her-2/neu through the action of PEA3 protein at the promoter level may represent a novel mechanism linking "Mediterranean diet" and cancer.

PMID: 16406575 [PubMed - as supplied by publisher]

Interesting.

Do you have a link for this. Is the full trial available?

I am sure you have noted that this trial per the available information is for oleic acid, and not olive oil. Oleic acid is a significant constituent in quality olive oils, but the omega six must be a factor too - what is the combined effect etc.

I wish they would do trials that looked at the 3s, 6s, 9s on the same grounds and look at the differences and communalities. Does omega nine work by displacing six?


RB

1: Eur J Cancer. 2006 Jan 4; [Epub ahead of print]
stands for the European Journal of Cancer January 4, 2006 issue. I would go to their website and see if it is available for free or needs $$$ to view the full article.

Hope this helps

[CLTann]

I would like to clarify the misconception many people understand about oil and acid. All edible oils are triglycerides of fatty acids, which have different chain length, number or position of unsaturation, cis or trans forms etc. Fatty acids are not consumed by people as food, but are used in making industrial products such as paints and cosmetics. So please understand that the labels of oil or food regarding their ingredients are actually the glycerides of these acids. I am a research fellow on fatty acids and should know what I am talking about.


Ann

Thank you for your clarification. I am sorry if it was me you were commenting on. I know I have a great deal to learn. I am always pleased to be corrected.

Could you help more?

Is there a good book?

I have read the posts below, although I will have to read them a few more times.

Does the trial above mean it used triglicerides made up soley with Oleic acid.
Do the combinations of fats on the triglicerides form in patterns or is any combination at or possible.
Is it possible to isolate particular trigliceride groups for the purposes of these trials eg all three arms oleic.
Are they using triglicerides or acids for these trials and what does that mean in practical terms.
Can the body make oleic derivatives.

Is a lipid a trigliceride?

Many thanks

RB



http://biology.clc.uc.edu/courses/bio104/lipids.htm

http://www.healthgoods.com/Education...glycerides.htm