Is this nonsense or really amazing?

Rich66 · 09-12-2008, 03:03 PM

See more info in articles of interest section. Here is the pubmed listing. Says humans(not mice) were treated. Is this for real???

Int J Cancer. 2008 Jan 15;122(2):461-7.

Links
Immunotherapy of metastatic breast cancer patients with vitamin D-binding protein-derived macrophage activating factor (GcMAF).

Yamamoto N, Suyama H, Yamamoto N, Ushijima N.
Division of Cancer Immunology and Molecular Biology, Socrates Institute for Therapeutic Immunology, Philadelphia, PA 19126-3305, USA. nobutoyama@verizon.net
Serum vitamin D3-binding protein (Gc protein) is the precursor for the principal macrophage activating factor (MAF). The MAF precursor activity of serum Gc protein of breast cancer patients was lost or reduced because Gc protein was deglycosylated by serum alpha-N-acetylgalactosaminidase (Nagalase) secreted from cancerous cells. Patient serum Nagalase activity is proportional to tumor burden. The deglycosylated Gc protein cannot be converted to MAF, resulting in no macrophage activation and immunosuppression. Stepwise incubation of purified Gc protein with immobilized beta-galactosidase and sialidase generated probably the most potent macrophage activating factor (termed GcMAF) ever discovered, which produces no adverse effect in humans. Macrophages treated in vitro with GcMAF (100 pg/ml) are highly tumoricidal to mammary adenocarcinomas. Efficacy of GcMAF for treatment of metastatic breast cancer was investigated with 16 nonanemic patients who received weekly administration of GcMAF (100 ng). As GcMAF therapy progresses, the MAF precursor activity of patient Gc protein increased with a concomitant decrease in serum Nagalase. Because of proportionality of serum Nagalase activity to tumor burden, the time course progress of GcMAF therapy was assessed by serum Nagalase activity as a prognostic index. These patients had the initial Nagalase activities ranging from 2.32 to 6.28 nmole/min/mg protein. After about 16-22 administrations (approximately 3.5-5 months) of GcMAF, these patients had insignificantly low serum enzyme levels equivalent to healthy control enzyme levels, ranging from 0.38 to 0.63 nmole/min/mg protein, indicating eradication of the tumors. This therapeutic procedure resulted in no recurrence for more than 4 years. Copyright 2007 Wiley-Liss, Inc.
PMID: 17935130 [PubMed - indexed for MEDLINE

Rich66 · 09-12-2008, 04:25 PM

Have folks already seen and debunked this? Can you forward it to your onc for a response?The silence is deafening.

Rich66 · 09-13-2008, 01:01 PM

Sorry..gotta bump.

Andrea Barnett Budin · 09-13-2008, 01:11 PM

Rich, My oncological nutritionist has had me on Vit D3 -- 1,000 mg per day for the last yr.

You can read my signature for details of my story since '95. I have also listed my complete vitamin supplement list somewhere on this board.

Any questions, ask away, please...

Andi

Rich66 · 09-13-2008, 01:40 PM

I think this is different from vi D supplements.
"Stepwise incubation of purified Gc protein with immobilized beta-galactosidase and sialidase generated probably the most potent macrophage activating factor (termed Gc-MAF) ever discovered, which produces no adverse effect in humans."

"weekly administration of Gc-MAF (100 ng)."

Andrea Barnett Budin · 09-13-2008, 02:09 PM

Major discovery regarding metastasis prevention in bc
http://www.medscape.com/viewarticle/570449

Lani posted the above a while back

+ see Lani post of 10/16/07 re this topic

Andi

Rich66 · 09-13-2008, 02:34 PM

Ok..as I reread for the millionth time...
Perhaps the issue here is how they describe "metastatic" as having high nagalese, indicating mets with reduction indicating tumor eradication. No mention of actual metastatic tumors to observe. Or am I missing something?

Andrea Barnett Budin · 09-13-2008, 06:51 PM

Is there a propellor head around who can help Rich out?

Andi

dorisranftl · 09-14-2008, 06:11 AM

Can someone tell me what this means in laymen's terms?

fullofbeans · 09-15-2008, 03:57 PM

Thanks Rich66 for posting.

I think it looks fantastic and from trustworthy scientific publication house.

The serum factor vitamin D3 binding protein (also called a Gc protein) is a naturally-produced molecule with a sugar component and a protein component. It is an abundant glyco-protein found in human blood serum [1]

What has been found a while ago [2] is that macrophage activating factor (GcMAF) was formed from vitamin D3 -binding protein. GcMAF greatly enhance phagocytic activity (i.e. immune cells killing ability).

Cancer cells secrete an enzyme known as alpha-N-acetylgalactosaminidase (also called Nagalase) that completely blocks conversion of Gc protein to MAF and therefore the cancer cells escape destruction by the immune system. But also makes people weaker and more susceptible to infection..

Anyhow what they can do (in the lab) is to transform the Gc protein (by stepwise incubation with 2 different enzyme) to produce GcMAF. Then they innoculate this enzymatically generated macrophage activating factor directly into the animal/human.

Ok I have not read the paper yet but it looks awesome what I am uncertain about is how the immune system knows what to destroy i.e. recognition of cancer cell is an issue and the founding principle of this her2 group i.e over expressing Her2 protein. But hey I could do with some GcMAF with my herceptin I reckon..

Best wishes

1-"The Gc protein (human group-specific component (Gc), a vitamin D-binding protein or Gc globulin), has important physiological functions that include involvement in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5a for neutrophils in inflammation and macrophage activation (mediated by a GalNAc-modified Gc protein (GcMAF)"
2- http://www.nature.com/icb/journal/v7...icb199833a.pdf