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R.B. · 07-12-2006, 02:40 PM

And another link to reproduction - growth factors involved at a very fundamanental level.

http://www.ncbi.nlm.nih.gov/entrez/q...=pubmed_DocSum

http://www.ncbi.nlm.nih.gov/entrez/q...=pubmed_DocSum

1: Dev Biol. 1999 Nov 15;215(2):399-406.Click here to read Links
Members of the ErbB receptor tyrosine kinases are involved in germ cell development in fetal mouse gonads.

* Toyoda-Ohno H,
* Obinata M,
* Matsui Y.

Department of Cell Biology, Institute of Development, Aging, and Cancer, Tohoku University, 4-1, Seiryo-machi, Sendai, Miyagi, 980-8575, Japan.

To isolate the genes involved in mouse primordial germ cell (PGC) development, we carried out subtraction cDNA cloning between PGC-derived embryonic germ (EG) cells and inner cell mass-derived embryonic stem cells. Among the genes preferentially expressed in EG cells, we found a gene encoding a receptor tyrosine kinase ErbB3. By in situ hybridization and immunohistochemical staining, the expression of ErbB3 as well as that of ErbB2, a coreceptor for ErbB3, was detected in PGCs in genital ridges at 12.5 dpc (days postcoitum). The expression was, however, downregulated at 14.5 dpc when the PGCs underwent growth cessation. Neuregulin-beta, a ligand for ErbB2 and ErbB3, was also expressed in genital ridges. In addition, a recombinant Neuregulin-beta enhanced the number of PGCs in 12.5-dpc embryos in culture. Taken together, these observations suggest that ErbB signaling controls the growth or survival of PGCs in genital ridges. Copyright 1999 Academic Press.

PMID: 10545246 [PubMed - indexed for MEDLINE]

07-12-2006, 02:40 PM	#6
R.B. Senior Member Join Date: Mar 2006 Posts: 1,843	And another link to reproduction - growth factors involved at a very fundamanental level. http://www.ncbi.nlm.nih.gov/entrez/q...=pubmed_DocSum http://www.ncbi.nlm.nih.gov/entrez/q...=pubmed_DocSum 1: Dev Biol. 1999 Nov 15;215(2):399-406.Click here to read Links Members of the ErbB receptor tyrosine kinases are involved in germ cell development in fetal mouse gonads. * Toyoda-Ohno H, * Obinata M, * Matsui Y. Department of Cell Biology, Institute of Development, Aging, and Cancer, Tohoku University, 4-1, Seiryo-machi, Sendai, Miyagi, 980-8575, Japan. To isolate the genes involved in mouse primordial germ cell (PGC) development, we carried out subtraction cDNA cloning between PGC-derived embryonic germ (EG) cells and inner cell mass-derived embryonic stem cells. Among the genes preferentially expressed in EG cells, we found a gene encoding a receptor tyrosine kinase ErbB3. By in situ hybridization and immunohistochemical staining, the expression of ErbB3 as well as that of ErbB2, a coreceptor for ErbB3, was detected in PGCs in genital ridges at 12.5 dpc (days postcoitum). The expression was, however, downregulated at 14.5 dpc when the PGCs underwent growth cessation. Neuregulin-beta, a ligand for ErbB2 and ErbB3, was also expressed in genital ridges. In addition, a recombinant Neuregulin-beta enhanced the number of PGCs in 12.5-dpc embryos in culture. Taken together, these observations suggest that ErbB signaling controls the growth or survival of PGCs in genital ridges. Copyright 1999 Academic Press. PMID: 10545246 [PubMed - indexed for MEDLINE]