HER2 Support Group Forums - View Single Post - for those with brain mets (and those scared of developing brain metastases)

R.B. · 07-07-2006, 04:10 AM

I posted this as part of the thread on the Greek diet as it relates to Omega threes.

"Taken together, these results indicate that omega-3 PUFA regulate COX-2-mediated invasion in brain-metastatic melanoma."

I have also posted the question on a number of occasions does Herceptin which is reported as intervening in the fat pathways block the making of DHA and EPA.

IF it does it would be a least in part a possible explanation as to a potential for increase of brain tumours.

It has been suggested that the balance of fats in the brain and risk of tumours are linked.

Smart Fats by A Schmidt ia an exellent book which gives an insight into the power and importance of fats. I do not know or have any connection with the persons involved in the book I have simply read it.

I repost this in the hoping of tempting those of you who have not looked at the issue of balancing the omega threes and sixes, and wider dietary issues to do so.

RB

http://www.ncbi.nlm.nih.gov/entrez/...l=pubmed_docsum

Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA. ydenkins@vetmed.lsu.edu

Cyclooxygenase-2 (COX-2) is important in the progression of epithelial tumors. Evidence indicates that omega-6 PUFAs such as arachidonic acid (AA) promote the growth of tumor cells; however, omega-3 fatty acids [eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)] inhibit tumor cell proliferation. We investigated the effects of omega-3 PUFA on the expression and function of COX-2 in 70W, a human melanoma cell line that metastasizes to the brain in nude mice. We show that 1) tumor necrosis factor-alpha upregulates the expression of both COX-2 mRNA and prostaglandin E2 (PGE2) production, and 2) omega-3 and omega-6 PUFA regulate COX-2 mRNA expression and PGE2 production. AA increased COX-2 mRNA expression and prostaglandin production in omega-6-stimulated 70W cells. Conversely, COX-2 mRNA expression decreased in cells incubated with EPA or DHA. AA increased Matrigel invasion 2.4-fold, whereas EPA or DHA did not. Additionally, PGE2 increased in vitro invasion 2.5-fold, whereas exposure to PGE3 significantly decreased invasion. Our results demonstrate that incubation of 70W cells with either AA or PGE2 increased invasiveness, whereas incubation with EPA or DHA downregulated both COX-2 mRNA and protein expression, with a subsequent decrease in Matrigel invasion. Taken together, these results indicate that omega-3 PUFA regulate COX-2-mediated invasion in brain-metastatic melanoma.

PMID: 15772428 [PubMed - indexed for MEDLINE]

07-07-2006, 04:10 AM	#7
R.B. Senior Member Join Date: Mar 2006 Posts: 1,843	I posted this as part of the thread on the Greek diet as it relates to Omega threes. "Taken together, these results indicate that omega-3 PUFA regulate COX-2-mediated invasion in brain-metastatic melanoma." I have also posted the question on a number of occasions does Herceptin which is reported as intervening in the fat pathways block the making of DHA and EPA. IF it does it would be a least in part a possible explanation as to a potential for increase of brain tumours. It has been suggested that the balance of fats in the brain and risk of tumours are linked. Smart Fats by A Schmidt ia an exellent book which gives an insight into the power and importance of fats. I do not know or have any connection with the persons involved in the book I have simply read it. I repost this in the hoping of tempting those of you who have not looked at the issue of balancing the omega threes and sixes, and wider dietary issues to do so. RB http://www.ncbi.nlm.nih.gov/entrez/...l=pubmed_docsum Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA. ydenkins@vetmed.lsu.edu Cyclooxygenase-2 (COX-2) is important in the progression of epithelial tumors. Evidence indicates that omega-6 PUFAs such as arachidonic acid (AA) promote the growth of tumor cells; however, omega-3 fatty acids [eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)] inhibit tumor cell proliferation. We investigated the effects of omega-3 PUFA on the expression and function of COX-2 in 70W, a human melanoma cell line that metastasizes to the brain in nude mice. We show that 1) tumor necrosis factor-alpha upregulates the expression of both COX-2 mRNA and prostaglandin E2 (PGE2) production, and 2) omega-3 and omega-6 PUFA regulate COX-2 mRNA expression and PGE2 production. AA increased COX-2 mRNA expression and prostaglandin production in omega-6-stimulated 70W cells. Conversely, COX-2 mRNA expression decreased in cells incubated with EPA or DHA. AA increased Matrigel invasion 2.4-fold, whereas EPA or DHA did not. Additionally, PGE2 increased in vitro invasion 2.5-fold, whereas exposure to PGE3 significantly decreased invasion. Our results demonstrate that incubation of 70W cells with either AA or PGE2 increased invasiveness, whereas incubation with EPA or DHA downregulated both COX-2 mRNA and protein expression, with a subsequent decrease in Matrigel invasion. Taken together, these results indicate that omega-3 PUFA regulate COX-2-mediated invasion in brain-metastatic melanoma. PMID: 15772428 [PubMed - indexed for MEDLINE] Last edited by R.B.; 07-07-2006 at 04:13 AM..