HER2 Support Group Forums - View Single Post

R.B. · 07-05-2006, 03:22 AM

I did a search on "fat syntesis P450" and this was on of the trials that came up.

This is a huge subject and every dooropened leads on to more. I do not pretend to understand the implications of this.

But I do not that it seems to suggest that P450 (Aromatase) may be involved in fat synthesis as well oestrogen regulation.

IF it is blocking aspects of fat synthesis it could have wider implications - see posts on omega three and six.

The impact of any potential blocking could be mitigated by ensuring that the body has an adequate intake of long chain fats particularly DAH and EPA.

Please discuss any dietary changes with your medical advisors.

RB

http://www.ncbi.nlm.nih.gov/entrez/q...=pubmed_docsum
1: J Pharmacol Exp Ther. 2006 May;317(2):732-8. Epub 2006 Jan 18. Related Articles, Links
Click here to read
Induction of renal cytochrome P450 arachidonic acid epoxygenase activity by dietary gamma-linolenic acid.

Yu Z, Ng VY, Su P, Engler MM, Engler MB, Huang Y, Lin E, Kroetz DL.

Department of Biopharmaceutical Sciences, University of California San Francisco, San Francisco, CA 94143, USA.

Dietary gamma-linolenic acid (GLA), a omega-6 polyunsaturated fatty acid found in borage oil (BOR), lowers systolic blood pressure in spontaneously hypertensive rats (SHRs). GLA is converted into arachidonic acid (AA) by elongation and desaturation steps. Epoxyeicosatrienoic acids (EETs) and 20-hydroxyeicosatetraenoic acid (20-HETE) are cytochrome P450 (P450)-derived AA eicosanoids with important roles in regulating blood pressure. This study tested the hypothesis that the blood pressure-lowering effect of a GLA-enriched diet involves alteration of P450-catalyzed AA metabolism. Microsomes and RNA were isolated from the renal cortex of male SHRs fed a basal fat-free diet for 5 weeks to which 11% by weight of sesame oil (SES) or BOR was added. There was a 2.6- to 3.5-fold increase in P450 epoxygenase activity in renal microsomes isolated from the BOR-fed SHRs compared with the SES-fed rats. Epoxygenase activity accounted for 58% of the total AA metabolism in the BOR-treated kidney microsomes compared with 33% in the SES-treated rats. More importantly, renal 14,15- and 8,9-EET levels increased 1.6- to 2.5-fold after dietary BOR treatment. The increase in EET formation is consistent with increases in CYP2C23, CYP2C11, and CYP2J protein levels. There were no differences in the level of renal P450 epoxygenase mRNA between the SES- and BOR-treated rats. Enhanced synthesis of the vasodilatory EETs and decreased formation of the vasoconstrictive 20-HETE suggests that changes in P450-mediated AA metabolism may contribute, at least in part, to the blood pressure-lowering effect of a BOR-enriched diet.

PMID: 16421287 [PubMed - indexed for MEDLINE]

07-05-2006, 03:22 AM	#12
R.B. Senior Member Join Date: Mar 2006 Posts: 1,843	I did a search on "fat syntesis P450" and this was on of the trials that came up. This is a huge subject and every dooropened leads on to more. I do not pretend to understand the implications of this. But I do not that it seems to suggest that P450 (Aromatase) may be involved in fat synthesis as well oestrogen regulation. IF it is blocking aspects of fat synthesis it could have wider implications - see posts on omega three and six. The impact of any potential blocking could be mitigated by ensuring that the body has an adequate intake of long chain fats particularly DAH and EPA. Please discuss any dietary changes with your medical advisors. RB http://www.ncbi.nlm.nih.gov/entrez/q...=pubmed_docsum 1: J Pharmacol Exp Ther. 2006 May;317(2):732-8. Epub 2006 Jan 18. Related Articles, Links Click here to read Induction of renal cytochrome P450 arachidonic acid epoxygenase activity by dietary gamma-linolenic acid. Yu Z, Ng VY, Su P, Engler MM, Engler MB, Huang Y, Lin E, Kroetz DL. Department of Biopharmaceutical Sciences, University of California San Francisco, San Francisco, CA 94143, USA. Dietary gamma-linolenic acid (GLA), a omega-6 polyunsaturated fatty acid found in borage oil (BOR), lowers systolic blood pressure in spontaneously hypertensive rats (SHRs). GLA is converted into arachidonic acid (AA) by elongation and desaturation steps. Epoxyeicosatrienoic acids (EETs) and 20-hydroxyeicosatetraenoic acid (20-HETE) are cytochrome P450 (P450)-derived AA eicosanoids with important roles in regulating blood pressure. This study tested the hypothesis that the blood pressure-lowering effect of a GLA-enriched diet involves alteration of P450-catalyzed AA metabolism. Microsomes and RNA were isolated from the renal cortex of male SHRs fed a basal fat-free diet for 5 weeks to which 11% by weight of sesame oil (SES) or BOR was added. There was a 2.6- to 3.5-fold increase in P450 epoxygenase activity in renal microsomes isolated from the BOR-fed SHRs compared with the SES-fed rats. Epoxygenase activity accounted for 58% of the total AA metabolism in the BOR-treated kidney microsomes compared with 33% in the SES-treated rats. More importantly, renal 14,15- and 8,9-EET levels increased 1.6- to 2.5-fold after dietary BOR treatment. The increase in EET formation is consistent with increases in CYP2C23, CYP2C11, and CYP2J protein levels. There were no differences in the level of renal P450 epoxygenase mRNA between the SES- and BOR-treated rats. Enhanced synthesis of the vasodilatory EETs and decreased formation of the vasoconstrictive 20-HETE suggests that changes in P450-mediated AA metabolism may contribute, at least in part, to the blood pressure-lowering effect of a BOR-enriched diet. PMID: 16421287 [PubMed - indexed for MEDLINE]