HER2 Support Group Forums - View Single Post - new blood test 100,000 more sensitive for her2neu than current serum her2neuECD ELISA

03-13-2006, 11:17 PM

EARLY VIEW: ABSTRACT: A sensitive and high-throughput assay to detect low-abundance proteins in serum [Nature Medicine; Subscribe; Sample]
The ability to detect antigens immunologically is limited by the affinity of the antibodies and the amount of antigens. We have now succeeded in creating a modular, facile amplification system, termed fluorescent amplification catalyzed by T7 polymerase technique (FACTT). Such a system can detect protein targets specifically at subfemtomolar levels (~0.08 fM). We describe here the detection of Her2 (also known as Neu) from rodent and human sera. FACTT is adaptable to high-throughput screening and automation and provides a practical method to enhance current ELISAs in medical practice.

03-13-2006, 11:17 PM	#2
Lani Guest Posts: n/a	for those who like to go to the source (sorry I do not know how to link) EARLY VIEW: ABSTRACT: A sensitive and high-throughput assay to detect low-abundance proteins in serum [Nature Medicine; Subscribe; Sample] The ability to detect antigens immunologically is limited by the affinity of the antibodies and the amount of antigens. We have now succeeded in creating a modular, facile amplification system, termed fluorescent amplification catalyzed by T7 polymerase technique (FACTT). Such a system can detect protein targets specifically at subfemtomolar levels (~0.08 fM). We describe here the detection of Her2 (also known as Neu) from rodent and human sera. FACTT is adaptable to high-throughput screening and automation and provides a practical method to enhance current ELISAs in medical practice.