HER2 Support Group Forums - View Single Post - results of ER, PR, her2 testing depend on whether specimen formalin-fixed promptly!!!

Lani · 09-09-2009, 07:47 AM

the oncotype dx relies on RNA for its results. This study should be repeated specifically looking for RNA degradation if formalin fixation is not performed promptly. Mammaprint and other microgene arrays are usually performed on fresh frozen samples. I wonder how:"fresh frozen" is defined? Within one minute of removal? Five minutes? one hour???

Lots of elephants in the room here!

09-09-2009, 07:47 AM	#3
Lani Senior Member Join Date: Mar 2006 Posts: 4,783	Re: results of ER, PR, her2 testing depend on whether specimen formalin-fixed promptl the oncotype dx relies on RNA for its results. This study should be repeated specifically looking for RNA degradation if formalin fixation is not performed promptly. Mammaprint and other microgene arrays are usually performed on fresh frozen samples. I wonder how:"fresh frozen" is defined? Within one minute of removal? Five minutes? one hour??? Lots of elephants in the room here!