HER2 Support Group Forums - View Single Post - Olive oil and Her2 downregulation

RhondaH · 01-28-2008, 02:49 AM

I found this article when I "originally" was not to get Herceptin and wanted to do what I could. In laymans terms, it was found that olive oil (46 % reduction of cell surface) provided "similar" results as Herceptin (48% reduction in cell surface), but TOGETHER they work synergistically (70% reduction in cell surface). SO I would drink up

http://www.ncbi.nlm.nih.gov/pubmed/1...ubmed_RVDocSum

RESULTS: Flow cytometric analyses demonstrated a dramatic (up to 46%) reduction of cell surface-associated p185(Her-2/neu) following treatment of the Her-2/neu-overexpressors BT-474 and SK-Br3 with OA. Indeed, this effect was comparable to that found following exposure to optimal concentrations of trastuzumab (up to 48% reduction with 20 microg/ml trastuzumab). Remarkably, the concurrent exposure to OA and suboptimal concentrations of trastuzumab (5 microg/ml) synergistically down-regulated Her-2/neu expression, as determined by flow cytometry (up to 70% reduction), immunoblotting, and immunofluorescence microscopy studies. The nature of the cytotoxic interaction between OA and trastuzumab revealed a strong synergism, as assessed by MTT-based cell viability and anchorage-independent soft-agar colony formation assays.

Rhonda

01-28-2008, 02:49 AM	#5
RhondaH Senior Member Join Date: Sep 2005 Location: Grand Rapids, MI Posts: 1,516	Here's one of the "original" olive oil and HER2 studies I found this article when I "originally" was not to get Herceptin and wanted to do what I could. In laymans terms, it was found that olive oil (46 % reduction of cell surface) provided "similar" results as Herceptin (48% reduction in cell surface), but TOGETHER they work synergistically (70% reduction in cell surface). SO I would drink up http://www.ncbi.nlm.nih.gov/pubmed/1...ubmed_RVDocSum RESULTS: Flow cytometric analyses demonstrated a dramatic (up to 46%) reduction of cell surface-associated p185(Her-2/neu) following treatment of the Her-2/neu-overexpressors BT-474 and SK-Br3 with OA. Indeed, this effect was comparable to that found following exposure to optimal concentrations of trastuzumab (up to 48% reduction with 20 microg/ml trastuzumab). Remarkably, the concurrent exposure to OA and suboptimal concentrations of trastuzumab (5 microg/ml) synergistically down-regulated Her-2/neu expression, as determined by flow cytometry (up to 70% reduction), immunoblotting, and immunofluorescence microscopy studies. The nature of the cytotoxic interaction between OA and trastuzumab revealed a strong synergism, as assessed by MTT-based cell viability and anchorage-independent soft-agar colony formation assays. Rhonda __________________ Rhonda Dx 2/1/05, Stage 1, 0 nodes, Grade 3, ER/PR-, HER2+ (3.16 Fish) 2/7/05, Partial Mastectomy 5/18/05 Finished 6 rounds of dose dense TEC (Taxotere, Epirubicin and Cytoxan) 8/1/05 Finished 33 rads 8/18/05 Started Herceptin, every 3 weeks for a year (last one 8/10/06) 2/1/13...8 year Cancerversary and I am "perfect" (at least where cancer is concerned;) " And in the end, it's not the years in your life that count. It's the life in your years."- Abraham Lincoln Last edited by RhondaH; 01-28-2008 at 02:55 AM.. Reason: addition