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R.B. · 10-04-2007, 03:00 PM

This is the result of a search on hempseed and canaboids. (See previous post)

I had previously seen suggestions that hempseed products could show up in drugs tests which had caused problems.

I have seen trials that suggest dietary benefit.

This site claims there are no adverse effects

http://www.sixwise.com/newsletters/0...the_planet.htm

As was suggested trials show some of the active ingredient in found in oils, foods etc.

What the implications are I do not know.

http://www.ncbi.nlm.nih.gov/sites/en...RVAbstractPlus

http://www.ncbi.nlm.nih.gov/sites/en...ubmed_RVDocSum

http://www.ncbi.nlm.nih.gov/sites/en...RVAbstractPlus

1: J Pharm Biomed Anal. 2005 Jan 4;36(5):939-46.Links
A rapid and simple procedure for the determination of cannabinoids in hemp food products by gas chromatography-mass spectrometry.
Pellegrini M, Marchei E, Pacifici R, Pichini S.

Drug Research and Control Department, Istituto Superiore di Sanitá, V.le Regina Elena 299, 00161 Rome, Italy. manuela.pellegrini@iss.it

A rapid and simple procedure using liquid-liquid extraction and subsequent gas chromatographic mass-spectrometric detection has been developed for determination of Delta9-tetrahydrocannabinol (THC), cannabidiol (CBD) and cannabinol (CBN) in different hemp foods. After addition of Delta8-tetrahydrocannabinol as internal standard, both solid and liquid specimens were extracted with two volumes of 2 ml of hexane/isopropanol (9:1): Chromatography was performed on a fused silica capillary column and analytes were determined in the selected-ion-monitoring (SIM) mode. The method was validated in the range 1-50 ng/ml liquid samples or 1-50 ng/g solid samples for THC and CBN, and 2-50 ng/ml or ng/g for CBD. Mean recoveries ranged between 78.8 and 90.2% for the different analytes in solid and liquid samples. The quantification limits were 1 ng/ml or ng/g for THC and CBN and 2 ng/ml or ng/g CBD. The method was applied to analysis of various hemp foods. THC content in different products varied 50-fold, whereas CBN and CBD were absent in some samples and achieved hundreds of ng/ml or ng/g in others. The concentration ratio (THC + CBN)/CBD was used to differentiate between the phenotypes of cannabis plants in different specimens. Products possibly originating from drug-type cannabis plants were found in the majority of analyzed specimens.

10-04-2007, 03:00 PM	#9
R.B. Senior Member Join Date: Mar 2006 Posts: 1,843	This is the result of a search on hempseed and canaboids. (See previous post) I had previously seen suggestions that hempseed products could show up in drugs tests which had caused problems. I have seen trials that suggest dietary benefit. This site claims there are no adverse effects http://www.sixwise.com/newsletters/0...the_planet.htm As was suggested trials show some of the active ingredient in found in oils, foods etc. What the implications are I do not know. http://www.ncbi.nlm.nih.gov/sites/en...RVAbstractPlus http://www.ncbi.nlm.nih.gov/sites/en...ubmed_RVDocSum http://www.ncbi.nlm.nih.gov/sites/en...RVAbstractPlus 1: J Pharm Biomed Anal. 2005 Jan 4;36(5):939-46.Links A rapid and simple procedure for the determination of cannabinoids in hemp food products by gas chromatography-mass spectrometry. Pellegrini M, Marchei E, Pacifici R, Pichini S. Drug Research and Control Department, Istituto Superiore di Sanitá, V.le Regina Elena 299, 00161 Rome, Italy. manuela.pellegrini@iss.it A rapid and simple procedure using liquid-liquid extraction and subsequent gas chromatographic mass-spectrometric detection has been developed for determination of Delta9-tetrahydrocannabinol (THC), cannabidiol (CBD) and cannabinol (CBN) in different hemp foods. After addition of Delta8-tetrahydrocannabinol as internal standard, both solid and liquid specimens were extracted with two volumes of 2 ml of hexane/isopropanol (9:1): Chromatography was performed on a fused silica capillary column and analytes were determined in the selected-ion-monitoring (SIM) mode. The method was validated in the range 1-50 ng/ml liquid samples or 1-50 ng/g solid samples for THC and CBN, and 2-50 ng/ml or ng/g for CBD. Mean recoveries ranged between 78.8 and 90.2% for the different analytes in solid and liquid samples. The quantification limits were 1 ng/ml or ng/g for THC and CBN and 2 ng/ml or ng/g CBD. The method was applied to analysis of various hemp foods. THC content in different products varied 50-fold, whereas CBN and CBD were absent in some samples and achieved hundreds of ng/ml or ng/g in others. The concentration ratio (THC + CBN)/CBD was used to differentiate between the phenotypes of cannabis plants in different specimens. Products possibly originating from drug-type cannabis plants were found in the majority of analyzed specimens.