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Rich66 · 11-04-2009, 11:51 PM

Pomegranate Fruit Extract Impairs Invasion and Motility in Human Breast Cancer

Gazala N. Khan Department of Internal Medicine, University of Michigan,Ann Arbor, MI, USA, gkhan@umich.edu
Michael A. Gorin
Department of Internal Medicine, University of Michigan,Ann Arbor, MI, USA
Devin Rosenthal
Department of Internal Medicine, University of Michigan,Ann Arbor, MI, USA
Quintin Pan
Department of Internal Medicine, University of Michigan,Ann Arbor, MI, USA
Li Wei Bao
Department of Internal Medicine, University of Michigan,Ann Arbor, MI, USA
Zhi Fen Wu
Department of Internal Medicine, University of Michigan,Ann Arbor, MI, USA
Robert A. Newman
Department of Experimental Therapeutics, M.D. Anderson Cancer Center, Houston,TX, USA, Punisyn Pharmaceuticals, Haifa, Israel
Alison D. Pawlus
Department of Experimental Therapeutics, M.D. Anderson Cancer Center, Houston,TX, USA
Peiying Yang
Department of Experimental Therapeutics, M.D. Anderson Cancer Center, Houston,TX, USA
Ephraim P. Lansky
Punisyn Pharmaceuticals, Haifa, Israel
Sofia D. Merajver
Department of Internal Medicine, University of Michigan,Ann Arbor, MI, USA
Purpose. Pomegranate fruit extracts (PFEs) possess polyphenolic and other compounds with antiproliferative, pro-apoptotic and anti-inflammatory effects in prostate, lung, and other cancers. Because nuclear transcription factor-kB (NF-kB) is known to regulate cell survival, proliferation, tumorigenesis, and inflammation, it was postulated that PFEs may exert anticancer effects at least in part by modulating NF-kB activity. Experimental design. The authors investigated the effect of a novel, defined PFE consisting of both fermented juice and seed oil on the NF-kB pathway, which is constitutively active in aggressive breast cancer cell lines. The effects of the PFE on NF-kB—regulated cellular processes such as cell survival, proliferation, and invasion were also examined. Results. Analytical characterization of the bioactive components of the PFE revealed active constituents, mainly ellagitannins and phenolic acids in the aqueous PFE and conjugated octadecatrienoic acids in the lipid PFE derived from seeds.The aqueous PFE dose-dependently inhibited NF-kB—dependent reporter gene expression associated with proliferation, invasion, and motility in aggressive breast cancer phenotypes while decreasing RhoC and RhoA protein expression. Conclusion. Inhibition of motility and invasion by PFEs, coincident with suppressed RhoC and RhoA protein expression, suggests a role for these defined extracts in lowering the metastatic potential of aggressive breast cancer species.

Key Words: breast cancer • cancer prevention • in vitro studies • Punica granatum

Integrative Cancer Therapies, Vol. 8, No. 3, 242-253 (2009)
DOI: 10.1177/1534735409341405

11-04-2009, 11:51 PM	#2
Rich66 Senior Member Join Date: Feb 2008 Location: South East Wisconsin Posts: 3,431	Re: Pomegranate fruit extract impairs invasion and motility in human breast cancer Pomegranate Fruit Extract Impairs Invasion and Motility in Human Breast Cancer Gazala N. Khan Department of Internal Medicine, University of Michigan,Ann Arbor, MI, USA, gkhan@umich.edu Michael A. Gorin Department of Internal Medicine, University of Michigan,Ann Arbor, MI, USA Devin Rosenthal Department of Internal Medicine, University of Michigan,Ann Arbor, MI, USA Quintin Pan Department of Internal Medicine, University of Michigan,Ann Arbor, MI, USA Li Wei Bao Department of Internal Medicine, University of Michigan,Ann Arbor, MI, USA Zhi Fen Wu Department of Internal Medicine, University of Michigan,Ann Arbor, MI, USA Robert A. Newman Department of Experimental Therapeutics, M.D. Anderson Cancer Center, Houston,TX, USA, Punisyn Pharmaceuticals, Haifa, Israel Alison D. Pawlus Department of Experimental Therapeutics, M.D. Anderson Cancer Center, Houston,TX, USA Peiying Yang Department of Experimental Therapeutics, M.D. Anderson Cancer Center, Houston,TX, USA Ephraim P. Lansky Punisyn Pharmaceuticals, Haifa, Israel Sofia D. Merajver Department of Internal Medicine, University of Michigan,Ann Arbor, MI, USA Purpose. Pomegranate fruit extracts (PFEs) possess polyphenolic and other compounds with antiproliferative, pro-apoptotic and anti-inflammatory effects in prostate, lung, and other cancers. Because nuclear transcription factor-kB (NF-kB) is known to regulate cell survival, proliferation, tumorigenesis, and inflammation, it was postulated that PFEs may exert anticancer effects at least in part by modulating NF-kB activity. Experimental design. The authors investigated the effect of a novel, defined PFE consisting of both fermented juice and seed oil on the NF-kB pathway, which is constitutively active in aggressive breast cancer cell lines. The effects of the PFE on NF-kB—regulated cellular processes such as cell survival, proliferation, and invasion were also examined. Results. Analytical characterization of the bioactive components of the PFE revealed active constituents, mainly ellagitannins and phenolic acids in the aqueous PFE and conjugated octadecatrienoic acids in the lipid PFE derived from seeds.The aqueous PFE dose-dependently inhibited NF-kB—dependent reporter gene expression associated with proliferation, invasion, and motility in aggressive breast cancer phenotypes while decreasing RhoC and RhoA protein expression. Conclusion. Inhibition of motility and invasion by PFEs, coincident with suppressed RhoC and RhoA protein expression, suggests a role for these defined extracts in lowering the metastatic potential of aggressive breast cancer species. Key Words: breast cancer • cancer prevention • in vitro studies • Punica granatum Integrative Cancer Therapies, Vol. 8, No. 3, 242-253 (2009) DOI: 10.1177/1534735409341405 __________________ Mom's treatment history (link)