Thread: Mushrooms
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Old 01-30-2011, 12:23 AM   #29
Jackie07
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Re: Mushrooms

The most recent report related to ganoderma lucidum:

Oncol Rep. 2011 Jan;25(1):267-72.
A novel polysaccharide from Se-enriched Ganoderma lucidum induces apoptosis of human breast cancer cells.

Shang D, Li Y, Wang C, Wang X, Yu Z, Fu X.
Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, College of Life Science, Liaoning Normal University, Dalian, P.R. China. djshang@lnnu.edu.cn
Abstract

The novel polysaccharide SeGLP-2B-1 isolated from Se-enriched Ganoderma lucidum, showed anti-proliferative activity towards several cancer cell lines in vitro. To investigate the antitumor mechanisms, the apoptotic effects of SeGLP-2B-1 in human breast cancer cells were studied, and the mechanism of this action was further elucidated. Cell apoptosis was detected by Annexin V/PI staining. Caspase activity was assayed using a caspase apoptosis detection kit. Western blot analysis was used to evaluate the levels of pro-caspase-3, -8, -9, PARP and cytochrome c expression. The results showed that SeGLP-2B-1 inhibited the growth of MCF-7 cells in a time- and dose-dependent manner. Typical characteristics of apoptosis were observed, including morphological changes, sub-G1 cells and DNA ladder formation. Further analysis showed that SeGLP-2B-1 treatment disrupted the mitochondrial membrane potential followed by an increase in the cytochrome c cytosolic levels. Sequentially, SeGLP-2B-1 increased the activities of caspase-9, -3 and poly (ADP-ribose) polymerase in a time-dependent manner, however, no obvious activation of caspase-8 was observed. Caspase-9 and caspase-3 inhibitor prevented SeGLP-2B-1-induced apoptosis, and the activities of caspases-3, -9 were significantly up-regulated by SeGLP-2B-1. Our studies suggest that SeGLP-2B-1 induces apoptosis via a mitochondria-mediated pathway.

PMID: 21109986 [PubMed - in process]
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