HER2 Support Group Forums - a cure for her2+ BCs--by making sure the puppy never gets born!

perhaps I should call it a cure/prevention.... if you prevent the causative switch from causing her2+ bd (birth of puppy) there is no puppy to escape to try to chase the squirrels!

works better than combo of herceptin, pertuzumab & cetuximab as the puppy never gets born in the first place rather than has to be contained sucessfully by being locked in the house by closing the front door, the back door and the window so it can't get out to chase the squirrels.

This is HUGE!(not just blocking the receptors so ligands cannot bind but preventing dimerization (the "holding of hands" of different her2s,her2s with her3s,her4s and egfrs) so the pathway never gets activated
ie those with the hands cannot join together create something greater than themselves & be able to to wreak havoc...

(((my "puppy" analogy posted previously:
Cancer is like a puppy dog that wants to get out of the house to chase the squirrels. Herceptin is like locking the front door. They puppy may learn it is possible to get out to play with the squirrels by going out the backdoor instead (eg, using her3, EGFR, IGFR, ER, etc paths to escape the blockade of the her2 pathway). Giving chemo is often like accelerating the aging of the puppy. It gets taller and able to jump on the dresser and jump out the window!!

For Stage IVs, it is usually just a question of what they will add next to herceptin if monotherapy is inssufficient to keep it from progressing(like which window/door to close in addition in the case of the puppy) )))

PUBLIC RELEASE: 27-APR-2015
Mayo Clinic-led research team identifies master switch for cancer-causing HER2 protein
MAYO CLINIC

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ROCHESTER, Minn. -- Herceptin has been touted as a wonder drug for women with HER2-positive breast cancer, an aggressive form of the disease that is fueled by excess production of the HER2 protein. However, not all of these patients respond to the drug, and many who do respond eventually acquire resistance.

A team of researchers led by Mayo Clinic has found a promising way to circumvent this obstacle. They identified a small site in the HER2 protein that enables it to form a molecular switch that sets off a cascade of events that turn normal cells cancerous. The researchers showed that disrupting this site can stem the growth of breast cancer cells, even more effectively than drugs currently used in the clinic. Their study is published in the Journal of the National Cancer Institute.

"This study is the first to look at the specific sequences for dimerization of HER2 as a possible anti-cancer target," says the study's senior author Ruth Lupu, Ph.D., a professor of experimental pathology and laboratory medicine and biochemistry and molecular biology at Mayo Clinic. "This finding could be beneficial not only for breast cancer, but also for other cancers with abnormal HER2 levels, such as ovarian, stomach and prostate cancer."

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One in every five breast cancers is HER2-positive, meaning that they have too many copies of the HER2 gene and/or produce too much of its product, the human epidermal growth factor receptor 2 (HER2) protein. A receptor like HER2 sits idle on the surface of the cell, becoming active only after pairing up with itself or other members of the protein family. These pairings create a kind of molecular key that opens up communication channels into the cell for stimulating proliferation and growth. When there is too much HER2 around, the protein pairs up even when it should not and that sends in a constant stream of signals telling the cell to grow out of control.

The discovery of HER2 and its role in breast cancer has led to the development of several therapies that specifically target its ability to transform cells. Trastuzumab (known commercially as Herceptin), pertuzumab and cetuximab have together significantly extended the lives of women with HER2-positive breast cancer. But none of the treatments has specifically targeted the ability of HER2 cells to join together or with other proteins, an essential first step in tumor growth.

Dr. Lupu hypothesized that HER2 activation occurs through a "functional site," a section of protein that is ultimately responsible for forming pairs with itself or other proteins of the same receptor family. If such a site exists, then blocking it would deactivate HER2, stopping tumor growth and metastasis. Dr. Lupu and her colleagues studied the protein sequence of HER2 and found a region that appeared to be involved in its dimerization.

They introduced a series of deletions into this region and eventually zeroed in on a section that contained just a short stretch of 16 amino acids, the building blocks of proteins. The researchers showed that unlike the wild-type HER2, a mutant protein that was missing this short sequence could not transform normal cells into cancer. Most importantly, when they added this mutant protein to HER2-positive breast cancer cells they showed that it halted the growth of these cells, overcoming the molecular makeup that made them aggressive. At the same time, they treated HER2-positive cells with the drugs trastuzumab, pertuzumab and cetuximab and found that the HER2-mutant outperformed all three HER2-targeted therapies.

"Our study demonstrates that this protein sequence is a druggable target," says Dr. Lupu. "Targeting this sequence could have a much broader impact than other drugs that are currently available because it does not just disrupt HER2, but it actually gets in the way of HER2's dimerization to itself and other family members. As a result, this approach could block many of the different pathways by which cancer-causing signals get sent into the cell."

Dr. Lupu and her colleagues are now confirming the anti-tumor activity of the HER2 mutant in relevant animal models, a necessary step before studies can move on to clinical testing. They are also investigating drugs -- such as mimetic agents, targeted antibodies and small molecules -- that could specifically block this site responsible for HER2's oncogenic potential.

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Co-authors of the paper include Barbara Schroeder, Ph.D. of Mayo Clinic, Javier Menendez, Catalan, Institute of Oncology, Susan Peirce, Ph.D., Emory University Medical School, Luciano Vellon, Ph.D., University of Buenos Aires School of Medicine, Adriana Papadimitropoulou, Laboratory of Molecular Biology & Immunobiotechnology, Hellenic Pasteur Institute and Ingrid Espinoza Ph.D., University of Mississippi Medical Center.

The work was supported by the U.S. Army Medical Research and Materiel Command (W81XWH-06-1-0686).

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As a leading institution funded by the National Cancer Institute, Mayo Clinic Cancer Center conducts basic, clinical and population science research, translating discoveries into improved methods for prevention, diagnosis, prognosis and therapy. For information on cancer clinical trials, call 1-855-776-0015 (toll-free).

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