human papillloma virus and breast cancer (and her2 overexpression in HPV+ pap smears)

[Lani]

periodically papers come out about eb virus, mouse mammary tumor virus and HPV found in breast cancer. here is one--and I will then post one on her2 overexpression in HPV+ pap smears. Food for thought

J Exp Clin Cancer Res. 2006 Dec;25(4):515-21.
HPV DNA frequency and subset analysis in human breast cancer patients' normal and tumoral tissue samples.

Gumus M,
Yumuk PF,
Salepci T,
Aliustaoglu M,
Dane F,
Ekenel M,
Basaran G,
Kaya H,
Barisik N,
Turhal NS.
Department of Internal Medicine, Division of Medical Oncology, Dr. Lutfi Kirdar Kartal Research and Training Hospital, Istanbul, Turkey. mgumus@superonline.com
Viruses are known to be associated with human malignancies, e.g., Epstein-Barr virus, human papillomavirus (HPV) and human T-cell leukemia virus type I. We conducted a prospective study to define the role of HPV in breast cancer. The malignant and normal breast tissue samples of 50 consecutive breast cancer patients were obtained postoperatively. DNA extracted from all tissues was amplified with the polymerase chain reaction using HPV primers. HPV 11, 16, 18, 33 subtypes were searched in HPV-DNA positive samples. Thirty-seven samples (74%) of tumoral breast tissue expressed HPV-DNA, 16 normal breast tissue samples (32%) were positive as well. There was a significant difference in HPV-DNA positivity between normal and tumoral breast tissue samples. HPV 18 was detected in 20 of the HPV-DNA positive tumoral tissue (54.4%) and in 9 of the HPV-DNA positive normal tissue (56.3%). HPV-33 also was detected in 35 (94.6 %) of the HPV-DNA positive tumoral tissue and in 14 (87.5 %) of the HPV-DNA positive normal tissue samples. HPV DNA was significantly associated with breast tumor tissue compared to normal breast tissue. Additional studies looking at HPV and HPV subtypes are needed to clarify the etiological role of the HPV in breast cancer.
PMID: 17310842 [PubMed - in process]

[Lani]

Gynecol Oncol. 2007 Feb 14; [Epub ahead of print] Links
Gene profiling in Pap-cell smears of high-risk human papillomavirus-positive squamous cervical carcinoma.

Manavi M,
Hudelist G,
Fink-Retter A,
Gschwandtler-Kaulich D,
Pischinger K,
Czerwenka K.
Department of Gynecology and Obstetrics, Division of Special Gynecology, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria.
OBJECTIVE.: The purpose of the study was to investigate benign and malignant squamous cervical cells obtained by cervical swabs with regard to differentially expressed genes and gene expression profiling, in order to evaluate the biological behavior and clinical outcome of cervical malignancies. METHODS.: Cervical squamous cells from six women with high-risk human papillomavirus positive [HR-HPV(+)] cervical carcinoma and from six HPV-negative women with normal ectocervical cells were analyzed by cDNA array. RESULTS.: cDNA over-expression of several genes such as MET (c-met), Nm23-H1 (NME1), EGFR, KGFR, Nm23-H2 (NME2), ERBB2 (c-erbB-2), cyclin-dependent kinase inhibitor 4 (CDKN2A, p16(INK4A)), cytokeratin 8 (KRT8), KRAS (K-ras), FLT1, KGF (FGF7), BCL2-like 2 protein (BCL2L2), ERBB4, MYCN (N-myc), cyclin D1 (CCND1), KIT (c-kit), secreted phosphoprotein 1 (SPP1) and STAT1, was significant in cervical squamous cell carcinoma (CSCC). Gene expression was downregulated for 13 genes in CSCC, such as interleukin 1 alpha (IL1A), the transforming growth factor receptor beta superfamily (TGFbeta; TGFB), some members of the insulin-like growth factor binding proteins (IGFBPs) and the integrin family (ITGA6, ITGB1). CONCLUSION.: This study was focused on the gene expression profiling of HR-HPV(-) and (+) cervical squamous cells and CSCC obtained by cytobrush. We observed gene expression patterns and signaling pathways that permit the investigator to distinguish between benign squamous cervical cells and CSCC with and without HPV infection.
PMID: 17306351 [PubMed - as supplied by publisher]